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His-Tag Mouse Monoclonal Antibody(4E6)
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His-Tag Mouse Monoclonal Antibody(4E6) |
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Catalog |
XHY004M |
XHY004L |
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Quantity |
50µL |
100µL |
For research use only.
Applications: WB, IP, IF
Species Cross-Reactivity: N/A
Molecular Weight: N/A
Isotype: IgG1
Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Store : -20°C. Do not aliquot the antibody.
Recommended dilutions: WB: 1:3,000 IP:1:200 IF:1:1,000
Optimal dilutions should be determined by the end user.
Specificity: The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.
Background: A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His)
residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can
greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify,
detect, and purify polyhistidine fusion proteins in bacteria insect cells, and mammalian cells
Applications:WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation
ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep